Let d be a Turing degree, R[d] and Q[d] denote respectively classes of recursively enumerable (r.e.) and all degrees in which d is relatively enumerable. We proved in Ishmukhametov [1999] that there is a degree d containing differences of r.e.sets (briefly, d.r.e.degree) such that R[d] possess a least elementm $>$ 0. Now we show the existence of a d.r.e. d such that R[d] has no a least element. We prove also that for any REA-degree d below 0 $'$ the class (...) Q[d] cannot have a least element and more generally is not bounded below by a non-zero degree, except in the trivial cases. (shrink)

Examining various kinds of isolation phenomena in the Turing degrees, I show that there are, for every n>0, (n+1)-c.e. sets isolated in the n-CEA degrees by n-c.e. sets below them. For n≥1 such phenomena arise below any computably enumerable degree, and conjecture that this result holds densely in the c.e. degrees as well. Surprisingly, such isolation pairs also exist where the top set has high degree and the isolating set is low, although the complete situation for jump classes remains unknown.