Results for 'PCR'

6 found
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  1. Molecular Analysis and Expression of Bap Gene in Biofilm-Forming Multi-Drug-Resistant Acinetobacter Baumannii.Omid Azizi, Mohammad Reza Shakibaie & Fereshteh Shahcherghi - 2016 - Reports of Biochemistry and Molecular Biology 5 (1):1-8.
    Acinetobacter baumannii is commonly resistant to nearly all antibiotics due to presence of antibiotic resistance genes and biofilm formation. In this study we determined the presence of (...)
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  2.  76
    Susceptibility of Catheter-Related Klebsiella Pneumoniae Strains to Quaternary Ammonium Compounds Under Biofilm and Planktonic Conditions.Mohammad Reaza Shakibaie, Fatemeh Monirzadeh, Melika Gholamrezazadeh & Shalaleh Masoumi - 2018 - Canadian Journal Infection Control 33 (4):215-219.
    Background: The aim of this study was to evaluate the susceptibility of catheter-related Klebsiella pneumoniae isolates to two biocides (benzalkonium chloride and Deconex) under biofilm and (...)planktonic conditions. Methods: A total of 85 strains of K. pneumoniae were isolated from catheters of inpatients hospitalized in four hospitals in Kerman, Iran. Susceptibility to antibiotics and biocides under biofilm and planktonic growths was performed using the microdilution method. Antibiofilm activity of the biocides was determined by microtiter assay. Biofilm eradication was carried out at different periods of time. The presence of cepA and qacEΔ1 genes were detected by polymerase chain reaction (PCR). Results: We found that 15% (n = 12) of the isolates showed strong biofilm activity, 40% (n = 35) displayed moderate activity, 30% (n = 26) demonstrated weak activity, and 15% (n = 12) showed no attachment to microtiter wells. Both the biocides had profound inhibitory activities on planktonic cells (average minimum inhibitory concentration [MIC] 0.06 ± 0.2 mg/ml for Deconex and 0.03 ± 0.1 mg/ml for benzalkonium chloride). They exerted the least antibiofilm activity at a sub-MIC concentration of 0.015 mg/ml. The isolates that formed high biofilm also harboured the cepA gene. Furthermore, a considerable increase in MIC to piperacillin/tazobactam, tetracycline, and cefotaxime was observed for cells grown in biofilm conditions for 24 hours, but all the isolates were sensitive to colistin and tigecycline. These differences were statistically significant, with a p-value of < 0.05. Most of the biofilms were eradicated from the microtiter plate within 30 minutesexposure to these biocides. Conclusions: As the data indicates, benzalkonium chloride and Deconex have good potential as hospital disinfectants for catheter-related infections caused by K. pneumoniae in planktonic conditions. Antimicrobial stewardship programs must be performed weekly in our hospitals to improve the quality of antimicrobial use, reduce the use of antibiotics, and shorten the length of hospital stay without increasing mortality rates. (shrink)
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  3.  60
    Analysis of Amino Acid Substitution Mutations of gyrA and parC Genes in Clonal Lineage of Klebsiella Pneumoniae Conferring High-Level Quinolone Resistance.Mohammad Reza Shakibaie & Amin Nourozi - 2014 - Journal of Medical Microbiology and Infectious Diseases 2 (3):109-117.
    Background: Emergence Klebsiella pneumoniae resistant to quinolone antibiotics due to mutations in gyrA and parC genes created problem for treatment of patients in different hospitals in Iran. (...)
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  4. Molecular Characterization of Aerobic Heterohophic Bacteria Isolated From Petroleum Hydrocarbon Polluted Brackish Waters of Bodo Creeks, Rivers State Nigeria.Tersagh Ichor - 2014 - Open Journal of Ecology 4:715-722.
    Surface water sources in the oil producing Niger Delta region of Nigeria are highly susceptible to pollution by petroleum hydrocarbons and so it is important to understand (...)
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  5.  70
    Prevalence of Β-Lactamase Genes, Class 1 Integrons, Major Virulence Factors and Clonal Relationships of Multidrug-Resistant Pseudomonas Aeruginosa Isolated From Hospitalized Patients in Southeast of Iran.Hossein Sharifi Mansouri, Gholamreza Pouladfar, Mohammad Reza Shakibaie, Bahman Pourabbas, Jalal Mardaneh, Shahla & Hosein Sharifi - 2019 - Iranian Journal Basic Medical Sciences 22 (7):806-812.
    Objective(s): Pseudomonas aeruginosa is one of the most important nosocomial pathogens causing a high rate of mortality among hospitalized patients. Herein, we report the prevalence of (...)antibiotic resistance genes, class 1 integrons, major virulence genes and clonal relationship among multidrug- resistant (MDR) P. aeruginosa, isolated from four referral hospitals in the southeast of Iran. Materials and Methods: In this study, 208 isolates of P. aeruginosa were collected from four referral hospitals in southeast of Iran. Disk diffusion method was used to determine susceptibility to 13 antibacterial agents. AmpC was detected by phenotypic method and β-lactamase genes, virulence genes and class 1 integrons were detected by PCR. Clonal relationship of the isolates was determined by RAPD-PCR. Results: All the isolates were susceptible to polymyxin-B and colistin. Overall, 40.4% of the isolates were MDR, among which resistance to third generation cephalosporins, aminoglycosides, and carbapenems was 47.5%, 32.3% and 40%, respectively. None of the isolates was positive for blaNDM-1 genes, while 84.5% and 4.8% were positive for the blaIMP-1 and blaVIM, metallo-β-lactamase genes, respectively. Incidence of class 1 integrons was 95% and AmpC was detected in 33% of the isolates. Prevalence of exoA, exoS, exoU, pilB and nan1 were 98.8%, 44%, 26%, 8.3% and 33.3%, respectively. RAPD profiles identified four large clusters consisting of 77 isolates, and two small clusters and three singletons. Conclusion: The rate of MDR P. aeruginosa isolates was high in different hospitals in this region. High genetic similarity among MDR isolates suggests cross-acquisition of infection in the region. (shrink)
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  6. Molecular Characterization of lpxACD and pmrA/B Two-Component Regulatory System in the Colistin Resistance Acinetobacter Baumannii Clinical Isolates.Mohammad Reaza Shakibaie - 2020 - Gene Reports 21.
    Colistin is drug of choice for treatment of carbapenem resistant Acinetobacter baumannii infections, but increasing colistin resistance (Col-R) has been emerged across the globe. In this (...)study, we collected 187 A. baumannii isolates from specimens of 240 patients admitted to intensive care units (ICUs) of two hospitals in Kerman, Iran during 2017-2018. Among the isolates, four isogenic extensive drug-resistant (XDR) strains with Minimum Inhibitory Concentration (MIC) ≥4 µg/mL against colistin were selected for further study. All the Col-R isolates harbored an intrinsic blaOXA51 and blaOXA-23 carbapenemase genes. They were resistant to all antibiotic classes except tigecycline and ampicillin-sulbactam. The Col-R isolates were belonged to clonal complex 2, a new ST type 1752 and had identical high-quality RAPD-PCR fingerprints. Phylogenetic tree analysis of PmrA/B suggested that, the Col-R A. baumannii were emerged by endogenous mutations rather than acquisition of preexisting clone. In addition, DNA sequencing of the Col-R genes, showed three different nonsynonymous substitutions in LpxA (N136K), LpxC (P293Q) and PmrB transmembrane motif (V21F and S28R) of the strains 1 and 3. Interestingly, these strains showed high level MIC against colistin (MIC 32µg/mL). Analysis of gene expression by relative quantitative real-time PCR (qRT-PCR) revealed 8- and 7.1-folds increases in the transcription levels of pmrB and pmrC genes in the strain 1 when cells grown in the presence of 16 µg/mL colistin (p0.01). In conclusion, the above results provide valuable insights into the mechanism of Col-R in A. baumannii and the expressions of relative genes. (shrink)
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