This study has assessed the psychometric proprieties of the Mental Toughness Inventory within the context of Brazilian sports. About 12 professionals participated in the process of adapting and translating the scale to Brazilian Portuguese. Subjects were 575 athletes who answered the MTI and the 10-item Connor–Davidson Resilience Scale. Data were analyzed through confirmatory factor analysis, Cronbach's alpha, composite reliability, average variance extracted, Spearman correlation, and model invariance tests. Results from CFA showed adequate fit for the original 8-item structure of the (...) scale [Chi-square = 27.041; p = 0.078; normalized chi-square = 1.50; comparative fit index = 0.988; Tucker–Lewis Index = 0.981; root mean square error of approximation = 0.03 [0.00–0.05]; standardized root mean residual = 0.030] assessing mental toughness as a single factor and the scale presented satisfactory internal consistency. MT was correlated with resilience, age, and time of experience in the sport. The MTI has also shown partial measurement invariance for sex and complete invariance across sport types. It was concluded that the MTI is a suitable tool for assessing MT in the present sample of Brazilian athletes; this instrument has potential practical application for researchers and sports psychologists who seek to develop the well-being and performance of athletes. (shrink)