Twentieth-century theoretical efforts towards the articulation of general system properties came short of having the significant impact on biological practice that their proponents envisioned. Although the latter did arrive at preliminary mathematical formulations of such properties, they had little success in showing how these could be productively incorporated into the research agenda of biologists. Consequently, the gap that kept system-theoretic principles cut-off from biological experimentation persisted. More recently, however, simple theoretical tools have proved readily applicable within the context of systems (...) biology. In particular, examples reviewed in this paper suggest that rigorous mathematical expressions of design principles, imported primarily from engineering, could produce experimentally confirmable predictions of the regulatory properties of small biological networks. But this is not enough for contemporary systems biologists who adopt the holistic aspirations of early systemologists, seeking high-level organizing principles that could provide insights into problems of biological complexity at the whole-system level. While the presented evidence is not conclusive about whether this strategy could lead to the realization of the lofty goal of a comprehensive explanatory integration, it suggests that the ongoing quest for organizing principles is pragmatically advantageous for systems biologists. The formalisms postulated in the course of this process can serve as bridges between system-theoretic concepts and the results of molecular experimentation: they constitute theoretical tools for generalizing molecular data, thus producing increasingly accurate explanations of system-wide phenomena. (shrink)

The main purpose of this study is to probe into the human capacity of understanding systems and defects in human knowledge of the world. The study addresses the greyness levels and systems levels and explains why the world cannot be perceived as a purely white or black structure. It also clarifies why human knowledge of systems always remains grey. The investigation relies on logical and deductive reasoning and uses the theoretical foundations of systems thinking and Boulding’s systems hierarchy. The most (...) important argument that this study advances is that human knowledge, in any form or under any circumstances, is grey and incomplete and will remain grey. Because the notion of “perfect knowledge” is ambiguous given human epistemic limits, any proportion of knowledge is incomplete and prone to change. Less complexity could lead to more accurate predications, but even in the simplest forms of systems, reaching perfect knowledge seems to be an unwarranted claim. Furthermore, because our perception of past events is incomplete, we cannot predict the future with certainty, as a result of which both the past and the future appear grey to us. The world, as an integrated system, is neither black nor white, but it remains grey, and the systems partially recognized by humans are part of the grey world. Gaining knowledge and increasing discoveries only contribute to the grey systems that are already known. (shrink)