Tobacco mosaic virus has served as a model organism for pathbreaking work in plant pathology, virology, biochemistry and applied genetics for more than a century. We were intrigued by a photograph published in Phytopathology in 1934 showing that Tabasco pepper plants responded to TMV infection with localized necrotic lesions, followed by abscission of the inoculated leaves. This dramatic outcome of a biological response to infection observed by Francis O. Holmes, a virologist at the Rockefeller Institute for Medical Research, was used (...) to score plants for resistance to TMV infection. Our objective was to gain a better understanding of early to mid-twentieth century ideas of genetic resistance to viruses in crop plants. We investigated Holmes’ observation as a practical exercise in reworking an experiment, having been inspired by Pamela Smith’s innovative Making and Knowing Project. We had a great deal of difficulty replicating Holmes’ experiment, finding that biological materials and experimental customs change over time, in ways that ideas do not. Using complementary tools plus careful study and interpretation of the original text and figures, we were able to rework, yet only partially replicate, this experiment. Reading peer-reviewed manuscripts that cited Holmes’ 1934 report provided an additional level of insight into the interpretation and replication of this work in the decades that followed. From this, we touch on how experimental reworking can inform our strategies to address the reproducibility “crisis” in twenty-first century science. (shrink)

This chapter presents the historical-investigative approach used in science teaching. Both history and philosophy of science have come to a sophisticated understanding of the role that experiments play in the generation and establishment of scientific knowledge. This recent development, called the “experimental turn,” is discussed first. Next, this chapter analyzes how practical work has been discussed among science educators in recent decades. Based on such a broad perspective, the historical-investigative approach is linked to recent advancements in history and philosophy of (...) science and in science education. Several modifications of the historical-investigative approach are outlined in order to illustrate their particular objectives, potential, and richness. (shrink)

I argue in the paper that classical chemistry is a science predominantly concerned with material substances, both useful materials and pure chemical substances restricted to scientific laboratory studies. The central epistemological and methodological status of material substances corresponds with the material productivity of classical chemistry and its way of producing experimental traces. I further argue that chemist’s ‘pure substances’ have a history, conceptually and materially, and I follow their conceptual history from the Paracelsian concept of purity to the modern concept (...) of pure stoichiometric compounds. The history of the concept of ‘pure substances’ shows that modern chemists’ concept of purity abstracted from usefulness rather than being opposed to it. Thus modern chemists’ interest in pure chemical substances does not presuppose a concept of pure science. (shrink)

Abstract: The aim of this paper is to analyse and criticize the argument of the experimenters' regress proposed by Harry Collins in 1985. In order to do that, I begin with an introduction to the experiments that aimed to detect gravity waves performed by Joseph Weber during 1970, then I analyse and discuss both forms of the argument: the epistemological and the ontological. Finally, after giving an outline of a theory of experimental reproduction and an explication of the concept of (...) replication, I propose two ways of avoiding the experimenters' regress. (shrink)

Summary The purpose of this work is to translate some of Robert Boyle's chemical experiments into the terms of modern chemistry. Most of the reactions involve sensible qualities, since there are on it considerable helpful tracking descriptions like heating, hissing, colour changing, etc. For a long time in the history of science, this procedure was seen as an exercise in anachronism which should be avoided at all costs. Recently many scholars have demonstrated that chemical translation can assist with historical work (...) instead of causing confusion, and it may be very useful as a tool for the history of chemistry and for reproducing past chemical experiments. (shrink)

Those with knowledge about scientific instruments come from many different fields. Prominent among them are (1) collectors and dealers, (2) curators, (3) historians, (4) instrument makers, (5) philosophers, and (6) scientists (the order is alphabetical, not value-laden). The annual symposium of the Scientific Instrument Commission often brings members of each of these groups together, and they learn from one another. What follows are brief reflections on the activities of each group when its members consider instruments.