This article introduces the special issue on questions, questioning, and institutional practices. We begin by considering how questioning as a discursive practice is a central vehicle for constructing social worlds and reflecting existing ones. Then we describe the different ways questions and question have been defined, typologized, and critiqued, in general and within seven institutions including policing, the courts, medicine, therapy, research interviews, education, and mediated political exchanges. The introduction concludes with a preview of the articles in the special issue.