We investigate some basic descriptive set theory for countably based completely quasi-metrizable topological spaces, which we refer to as quasi-Polish spaces. These spaces naturally generalize much of the classical descriptive set theory of Polish spaces to the non-Hausdorff setting. We show that a subspace of a quasi-Polish space is quasi-Polish if and only if it is Π20 source in the Borel hierarchy. Quasi-Polish spaces can be characterized within the framework of Type-2 Theory of Effectivity as precisely the countably based spaces (...) that have an admissible representation with a Polish domain. They can also be characterized domain theoretically as precisely the spaces that are homeomorphic to the subspace of all non-compact elements of an ω-continuous domain. Every countably based locally compact sober space is quasi-Polish, hence every ω-continuous domain is quasi-Polish. A metrizable space is quasi-Polish if and only if it is Polish. We show that the Borel hierarchy on an uncountable quasi-Polish space does not collapse, and that the Hausdorff–Kuratowski theorem generalizes to all quasi-Polish spaces. (shrink)