Collecting, comparing, and computing molecular sequences are among the most prevalent practices in contemporary biological research. They represent a specific way of producing knowledge. This paper explores the historical development of these practices, focusing on the work of Margaret O. Dayhoff, Richard V. Eck, and Robert S. Ledley, who produced the first computer-based collection of protein sequences, published in book format in 1965 as the Atlas of Protein Sequence and Structure. While these practices are generally associated with the rise of (...) molecular evolution in the 1960s, this paper shows that they grew out of research agendas from the previous decade, including the biochemical investigation of the relations between the structures and function of proteins and the theoretical attempt to decipher the genetic code. It also shows how computers became essential for the handling and analysis of sequence data. Finally, this paper reflects on the relationships between experimenting and collecting as two distinct “ways of knowing” that were essential for the transformation of the life sciences in the twentieth century. (shrink)

In 1957, Francis Crick outlined a startling vision of life in which the great diversity of forms and shapes of macromolecules was encoded in the one-dimensional sequence of nucleic acids. This paper situates Crick's new vision in the debates of the 1950s about protein synthesis and gene action. After exploring the reception of Crick's ideas, it shows how they differed radically from a different model of protein synthesis which enjoyed wide currency in that decade. In this alternative model, advocated by (...) Linus Pauling and other luminaries, three-dimensional templates directed the folding of proteins. Even though it was always considered somewhat speculative, this theory was supported by a number of empirical results originating in different experimental systems. It was eventually replaced by a model in which the forms and shapes of macromolecules resulted solely from their amino acid sequence, dramatically simplifying the problem of protein synthesis which Crick was attempting to solve in 1957. (shrink)

ABSTRACT Historians of science, inasmuch as they are concerned with knowledges and practices rather than institutions, have tended of late to focus on case studies of common processes such as experiment and publication. In so doing, they tend to treat science as a single category, with various local instantiations. Or, alternatively, they relate cases to their specific local contexts. In neither approach do the cases or their contexts build easily into broader histories, reconstructing changing knowledge practices across time and space. (...) This essay argues that by systematically deconstructing the practices of science and technology and medicine (STM) into common, recurrent elements, we can gain usefully “configurational” views, not just of particular cases and contexts but of synchronic variety and diachronic changes, both short term and long. To this end, we can begin with the customary actors’ disciplines of early modern knowledge (natural philosophy, natural history, mixed mathematics, and experimental philosophy), which can be understood as elemental “ways of knowing and working,” variously combined and disputed. I argue that these same working knowledges, together with a later mode—synthetic experimentation and systematic invention—may also serve for the analysis of STM from the late eighteenth century to the present. The old divisions continued explicitly and importantly after circa 1800, but they were also “built into” an array of new sciences. This historiographic analysis can help clarify a number of common problems: about the multiplicity of the sciences, the importance of various styles in science, and the relations between science and technology and medicine. It suggests new readings of major changes in STM, including the first and second scientific revolutions and the transformations of biomedicine from the later twentieth century. It offers ways of recasting both microhistories and macrohistories, so reducing the apparent distance between them. And it may thus facilitate both more constructive uses of case studies and more innovative and acceptable longer histories. (shrink)

Among the many groups of scholars whose work now illuminates science, technology and medicine (STM), historians, it seems to me, have a key responsibility not just to elucidate change but to establish and explain variety. One of the big pictures we need is a model of the varieties of STM over time; one which does not presume the timeless existence of disciplines, or the distinctions between science, technology and medicine; a model which is both synchronic and diachronic, and both cognitive (...) and social. To that end, this brief paper presents a historical typology of STM from about 1700 to the present by focusing on four ‘ideal’ socio-cognitive types – four knowledge structures which correspond to four sets of social relations. To some extent these are period specific, but they do not have to be – hence, one may hope, the flexibility and usefulness of the model. (shrink)

ArgumentHere I analyze the anatomical thought of the French physician and naturalist Félix Vicq d'Azyr (1748–1794) in order to bring to light its importance in the development of comparative anatomy at the end of the eighteenth century. I argue that his work and career can be understood as an ambitious program for a radical reform of all biomedical sciences and a reorganization of this whole field around comparative anatomy, on the conceptual as well as the institutional level. In particular, he (...) recommended a close connection between anatomical and physiological studies, and a generalization of the comparative approach towards organs and functions in man and animals. This conception led him not only to reform the scope, the methods, the style of description, and the vocabulary in anatomy, but also to construct a new classification of living beings and to pursue a quest for laws of organization. This strategy was successful, since Vicq d'Azyr was able to promote his thought as well as his institutional position efficiently. The Revolution and his untimely death prevented him from achieving his program, but his attempt would serve as an example for younger scientists like Cuvier. (shrink)