Philosophical investigation in synthetic biology has focused on the knowledge-seeking questions pursued, the kind of engineering techniques used, and on the ethical impact of the products produced. However, little work has been done to investigate the processes by which these epistemological, metaphysical, and ethical forms of inquiry arise in the course of synthetic biology research. An attempt at this work relying on a particular area of synthetic biology will be the aim of this chapter. I focus on the reengineering of (...) metabolic pathways through the manipulation and construction of small DNA-based devices and systems synthetic biology. Rather than focusing on the engineered products or ethical principles that result, I will investigate the processes by which these arise. As such, the attention will be directed to the activities of practitioners, their manipulation of tools, and the use they make of techniques to construct new metabolic devices. Using a science-in-practice approach, I investigate problems at the intersection of science, philosophy of science, and sociology of science. I consider how practitioners within this area of synthetic biology reconfigure biological understanding and ethical categories through active modelling and manipulation of known functional parts, biological pathways for use in the design of microbial machines to solve problems in medicine, technology, and the environment. We might describe this kind of problem-solving as relying on what Helen Longino referred to as “social cognition” or the type of scientific work done within what Hasok Chang calls “systems of practice”. My aim in this chapter will be to investigate the relationship that holds between systems of practice within metabolic engineering research and social cognition. I will attempt to show how knowledge and normative valuation are generated from this particular network of practitioners. In doing so, I suggest that the social nature of scientific inquiry is ineliminable to both knowledge acquisition and ethical evaluations. (shrink)

The eminent French biologist and historian of biology, François Jacob, once notoriously declared “On n’interroge plus la vie dans les laboratoires”: laboratory research no longer inquires into the notion of ‘Life’. Nowadays, as David Hull puts it, “both scientists and philosophers take ontological reduction for granted… Organisms are ‘nothing but’ atoms, and that is that.” In the mid-twentieth century, from the immediate post-war period to the late 1960s, French philosophers of science such as Georges Canguilhem, Raymond Ruyer and Gilbert Simondon (...) returned to Jacob’s statement with an odd kind of pathos: they were determined to reverse course. Not by imposing a different kind of research program in laboratories, but by an unusual combination of historical and philosophical inquiry into the foundations of the life sciences (particularly medicine, physiology and the cluster of activities that were termed ‘biology’ in the early 1800s). Even in as straightforwardly scholarly a work as La formation du concept de réflexe aux XVIIe et XVIIIe siècles (1955), Canguilhem speaks oddly of “defending vitalist biology,” and declares that Life cannot be grasped by logic (or at least, “la vie déconcerte la logique”). Was all this historical and philosophical work merely a reassertion of ‘mysterian’, magical vitalism? In order to answer this question we need to achieve some perspective on Canguilhem’s ‘vitalism’, notably with respect to its philosophical influences such as Kurt Goldstein. (shrink)

This thesis investigates the structure of knowing, and it argues that existential trust is an epistemological category. The aim of the dissertation is to develop a view according to which all human activity is seen as an activity of a lived body, and in which the understanding of the structure of such activity is regarded as central for the solution even of epistemological problems. This view is not rooted in any one philosophical tradition, but circles around activity of the lived (...) body. It connects thinkers who in other respects belong to different "isms" in philosophy. Central to the dissertation are Aristotle, Dewey, Merleau-Ponty, Wittgenstein, Ryle, Anscombe, Polanyi, and Grene. Michael Polanyi's concept of tacit knowledge, and connected concepts like attend to, attend from, and subsidiary awareness, are presented. Different kinds of subsidiary awareness, not noted by Polanyi, are distinguished. It is also argued that Polanyi has not seen all the implicatons of his view that instruments can be interiorized and be part of the lived body. Conversely, parts of the normally lived body can be exteriorized. Nor has Polanyi seen that one has subsidiary awareness of oneself as a certain kind ofperson. This fact, in turn, is shown to have implications for the way we constitute ourselves as agents. Since we are engendered agents, we always attend from gender. In the last decade, the concept of trust has definitely entered epistemology. Mostly, it has been in terms of trusting testimony and/or testifier. This thesis wants to deepen that account. There is a more fundamental kind of trust, namely trust in oneself and trust in the world. It is called existential trust, and it is of epistemological importance, too. Existential trust, it is claimed, is necessary in all acts of knowing. Probably, this fact is hard to discover without having recourse to a distinction like that between attending from and attending to. Existential trust is shown in the way we attend from something. Observation and thinking are central epistemological categories, of course, but they should be supplemented by trust. Without trust they cannot perform any epistemological work. Linguistically, 'to know' is a state verb. Superficially, it describes only a state, not an activity. This fact, however, does not contradict the view of knowledge put forward in the dissertation. To know is to have a disposition to perform successfully either some kind of action, or to perform some kind of true assertive speech act. Basically, knowing is an activity. (shrink)

I seek to provide a systematic and comprehensive framework for the description and analysis of scientific practice—a philosophical grammar of scientific practice, ‘grammar’ as meant by the later Wittgenstein. I begin with the recognition that all scientific work, including pure theorizing, consists of actions, of the physical, mental, and ‘paper-and-pencil’ varieties. When we set out to see what it is that one actually does in scientific work, the following set of questions naturally emerge: who is doing what, why, and how? (...) More specifically, we must arrive at some coherent philosophical accounts of the following elements of scientific practice: the agent—free, embodied, and constantly in second-person interactions with other agents; the purposes and proximate aims of the agent; types of activities that the agent engages in; ontological principles necessarily presumed for the performance of particular activities; instruments and other resources that the agent pulls together for the performance of each activity. After sketching the general framework, I also give some illustrative contrasts between the more traditional descriptions of scientific practice and the kind of descriptions enabled by the proposed framework. (shrink)

A revisionist movement in Anglo-Saxon philosophy of science seeking to modulate the positivistic stress on formalized systems and to consider science as ongoing research in finite historical context strikes resonances with hermeneutical phenomenology , whose ontology likewise shifts the locus of truth from verification to discovery. Fusion of the two traditions is utilized to illuminate hitherto relatively unexplored facets of the logic and psychology of scientific discovery, as well as its ontology, here developed from the intentional intertwining of man and (...) nature and finding its locus in the linguistic historicity of fundamental scientific concepts. (shrink)

Research in animal behavior begins by identifying what animals are doing. In the course of observation, the observer comes to see animals as performing a particular activity. How does this process work? How cn we be certain that behavior is identified correctly? Wittgenstein offers an approach to these questions. looking at the uses of certainly rather than attempting to find rules that guarantee it. Here two stages in research are distinguished: first, watching animals, and second, reporting the results to other (...) scientists. Certainly about what animals are doing, has different uses at each stage. (shrink)