Population aging has brought great challenges to many regions throughout the world. Enhancing the sense of participation, access, and well-being of older adults is the goal of China’s aging development. This study, taking urban–rural difference as the entry point, examined the difference in subjective well-being between urban and rural older learners. A total of 2,007 older adults learners aged over 50 years were recruited in Zhejiang, Anhui, and Shandong Provinces in China, including 773 rural older adults and 1,234 urban older (...) adults. This study found that there was a significant positive correlation between senior learning and the subjective well-being of urban and rural older adult learners. Furthermore, there was a significant difference between the subjective well-being of urban and rural older adult learners’ and there was also an urban–rural difference between the effects of older adult learning on the subjective well-being. Based on the above findings, this study reveals the mechanism of the impact of older adult learning on subjective well-being of urban and rural older adults and gives relevant suggestions for improving the subjective well-being of urban and rural older learners. (shrink)